Rhizobium Lipooligosaccharides Rescue a Carrot Somatic Embryo Mutant.

A carrot somatic embryo mutant is rescued by chitinase.

At the nonpermissive temperature, somatic embryogenesis of the temperature-sensitive (ts) carrot cell mutant ts11 does not proceed beyond the globular stage. This developmental arrest can be lifted by the addition of proteins secreted by wild-type cells to the culture medium. From this mixture of secreted proteins, a 32-kD glycoprotein, designated extracellular protein 3 (EP3), that allows comp...

Novel regulation of heat shock genes during carrot somatic embryo development.

We have determined that somatic embryos of carrot exhibit a number of interesting and unusual properties when exposed to heat shock at different times in their development. Specifically, we have seen that mid-globular embryos can be arrested irreversibly in their development when heat-shocked, whereas all other stages of embryogenesis, both before and after this stage, are fully capable of norm...

Isolation of putative embryo - specifie embryos genesusing stress induction of carrot somatic

bryogenesis is usefu1 for isolating genes expressed during embryogenesis, as embryegenic competence and the expressien of certain embryo-specific genes increases with increased stress. Fluorescent differential display (FDD) was perfonmed to isolate stress-induced genes in carrot somatic embryos, and five genes were isolated as embryonic tissue-specific genes. One ofthese genes was expressed unt...

Synchronization of somatic embryogenesis in a carrot cell suspension culture.

Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were compose...

LLC 18 Embryo Rescue Sandra